APE 1 from New England Biolabs

Product Specs
ItemAPE 1
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0282S
FormatSupplied with 10X Reaction Buffer
Molecule NameAPE 1
Quantity1000 units
ApplicationsDNA Manipulation
Source/Expression SystemIsolated from a recombinant source
Concentration10,000 units/ml
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeEnzymes
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to cleave 20 pmol of a 34 mer oligonucleotide duplex containing a single AP site* in a total reaction volume of 10 µl in 1 hour at 37°C.

*An AP site is created by treating 20 pmol of a 34 mer oligonucleotide duplex containing a single uracil residue with 1 unit of Uracil-DNA Glycosylase (UDG) for 2 minutes at 37°C.

Storage:
Storage Temperature: -20°C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Development of a DNAzyme Walker for the Detection of APE1 in Living Cancer CellsAnalytical ChemistryOctober 10, 2023Jeffrey Tao, Hongquan Zhang, Michael Weinfeld, X. Chris Lecontaining a single AP site in a total reaction volume of 10 μL in 1 h at 37 °C.” (neb.ca/detail.php?id=m0282). One U/μL of APE1 (1 U/μL) is approximately 1.17 ng/μL (information provided by NEB upon inquiry). Incubation,

(2) DNA-translocation-independent role of INO80 remodeler in DNA damage repairsJournal of Biological ChemistryOctober 1, 2023Vladyslava Sokolova, Gahyun Lee, Amber Mullins, Preesha Mody, Shinya Watanabe, Dongyan Tanand 6-FAM label on 3′ of the 601 DNA on the same strand) and 5 nM human APE1 endonuclease (NEB, cat # M0282S) were included in each reaction, with or without 25 nM endogenous INO80-C complex, with or without 1

(3) Detecting recurrent passenger mutations in melanoma by targeted UV damage sequencingNature CommunicationsMay 11, 2023Kathiresan Selvam, Smitha Sivapragasam, Gregory M. K. Poon, John J. Wyrick(M0315S, NEB). The DNA was then digested with T4 endonuclease V (T4 PDG, M0308S, NEB) and AP endonuclease (M0282S, NEB) to create 3′-OH groups immediately upstream of the CPD lesions, and the resulting fragments were

(4) Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repairScience advancesMay 10, 2023Nguyen DD, Kim E, Le NT, Ding X, Jaiswal RK, Kostlan RJ, Nguyen TNT, Shiva O, Le MT, Chai W.buffer 4 for 15 min at room temperature, followed by treatment with 10 U of APE1 (New England Biolabs, M0282S) in 1× NEB buffer 4 at 37°C for 1 hour. Slides were then washed with PBS and gently immerse in prechilled

(5) Improved cytosine base editors generated from TadA variantsNature BiotechnologyMay 1, 2023Dieter K. Lam, Patricia R. Feliciano, Amena Arif, Tanggis Bohnuud, Thomas P. Fernandez, Jason M. Gehrke, Phil Grayson, Kin D. Lee, Manuel A. Ortega, Courtney Sawyer, et al.treated with human Alkyl Adenine Glycosylase (hAAG; NEB 0313S) followed by AP Endonuclease 1 (APE1; NEB M0282L) according to manufacturer’s instructions. The combination of hAAG and APE1 was used because of our

(6) Characterization of a Novel Thermostable DNA Lyase Used To Prepare DNA for Next-Generation SequencingChemical research in toxicologyFebruary 20, 2023Baljinnyam T, Conrad JW, Sowers ML, Chang-Gu B, Herring JL, Hackfeld LC, Zhang K, Sowers LC.repair enzymes uracil-DNA glycosylase (UDG, #M0280S), human apurinic/apyrimidinic endonuclease 1 (APE1, #M0282S), formamidopyrimidine DNA glycosylase (FPG, #M0240S) and E. coli DNA ligase (ligase, #M0205S), endonuclease

(7) DNA Base Excision Repair Intermediates Influence Duplex–Quadruplex EquilibriumMoleculesJanuary 18, 2023Mark L. Sowers, James W. Conrad, Bruce Chang-Gu, Ellie Cherryhomes, Linda C. Hackfeld, Lawrence C. Sowersgel electrophoresis. 4.2. Enzymes E. coli uracil DNA glycosylase (UDG, ca# M0280S), human AP endonuclease 1 (APE1, ca# M0282S), and human single-strand selective monofunctional uracil DNA glycosylase (hSMUG1, ca# M0336S) were

(8) Enhancer RNAs stimulate Pol II pause release by harnessing multivalent interactions to NELFNature CommunicationsDecember 1, 2022Vladyslava Gorbovytska et al.CL4111K, Epicentre) for 2 h at 60 °C and relinearized at the basic dSpacer furan with Ape 1 (15 U; M0282S, NEB, Ipswich, MA) for 2 h at 37 °C. The relinearized single-stranded DNA template was subjected to

(9) Complex genomic patterns of abasic sites in mammalian DNA revealed by a high-resolution SSiNGLe-AP methodNature CommunicationsOctober 5, 2022Ye Cai, Huifen Cao, Fang Wang, Yufei Zhang, Philipp Kapranovaddition of 1 nmol biotin-11-ddCTP (Jena Bioscience, NU-850-BIOX-L) and 20 U TdT (New England BioLabs, M0282) and incubation at 37 °C for 4 h. The reaction was denatured again at 95 °C for 30 s, and 20 U TdT was

(10) A combinatorial system to examine the enzymatic repair of multiply damaged DNA substratesNucleic acids researchJuly 22, 2022Hsu CW, Conrad JW, Sowers ML, Baljinnyam T, Herring JL, Hackfeld LC, Hatch SS, Sowers LC.ligase, cat# M0205S), Uracil DNA glycosylase (UDG, cat# M0280S), human AP endonuclease 1 (APE1, cat# M0282S), and a thermostable AP endonuclease IV (Tth, cat# M0294S) were purchased from New England Biolabs (NEB). Human

(11) In vitro eradication of abasic site-mediated DNAâ€“peptide/protein cross-links by Escherichia coli long-patch base excision repairJournal of Biological ChemistryJuly 1, 2022Cameron Bryan, Xiaoying Wei, Zhishuo Wang, Kun YangM0304S), Exo III (Cat. #: M0206S), Pol I (Cat. #: M0209S), ligase (Cat. #: M0205S), human APE1 (Cat. #: M0282S), uracil-DNA glycosylase (UDG, Cat. #: M0280S), and Nt.BstNBI (Cat. #: R0607S) were purchased from the

(12) Global-run on sequencing identifies Gm11967 as an Akt-dependent long noncoding RNA involved in insulin sensitivityiScienceJune 17, 2022Dominic Santoleri, Hee-Woong Lim, Matthew J. Emmett, Julian Stoute, Matthew J. Gavin, Jaimarie Sostre-ColÃ³n, Kahealani Uehara, Jaclyn E. Welles, Kathy Fange Liu, Mitchell A. Lazar, et al.recombinant proteins","Chemicals, peptides, and recombinant proteins" ,, APE I,New England Biolabs,M0282 CircLigase,Epicentre,Cat# CL4111K Collagenase Type 2,Worthington Biochemical,Cat# LS004174; CAS: 9001-12-1 Cycloheximide,Sigma-Aldrich,Cat#

(13) Human TDP1, APE1 and TREX1 repair 3'-DNA-peptide/protein cross-links arising from abasic sites in vitroNucleic acids researchApril 22, 2022Wei X, Wang Z, Hinson C, Yang K.Phusion® high-fidelity DNA polymerase (Cat. # M0530S), Quick Ligation™ Kit (Cat. # M2200S), Human APE1 (Cat. # M0282S) and exonuclease III (ExoIII, Cat. # M0206S) were purchased from the New England Biolabs. Human DNA

(14) XRCC1 Prevents Toxic PARP1 Trapping During DNA Base Excision RepairSSRN Electronic JournalMarch 31, 2022Annie A. Demin et al.(Adh) Sigma-Aldrich Cat#A3263 PARG enzyme Trevigen N/A Exonuclease III NEB Cat#M0206S APE1 NEB Cat#M0282S Uracil-DNA Glycosylase (UDG) NEB Cat#M0280S Recombinant PARP1 This paper N/A Recombinant XRCC1 This

(15) High-resolution mapping demonstrates inhibition of DNA excision repair by transcription factorseLifeMarch 15, 2022Mingrui Duan, Smitha Sivapragasam, Jacob S Antony, Jenna Ulibarri, John M Hinz, Gregory M K Poon, John J Wyrick, Peng Maofree 3’ ends (Ding et al., 2015). The NMP lesion site was cleaved by hAAG (NEB, M0313S) and APE1 (NEB, M0282S) to generate a new ligatable 3’ end. DNA was denatured at 95°C and cooled on ice, followed by ligation

(16) Versatile cell-based assay for measuring DNA alkylation damage and its repairScientific ReportsDecember 1, 2021Yong Li, Peng Mao, Evelina Y. Basenko, Zachary Lewis, Michael J. Smerdon, Wioletta Czajanitrogen. Genomic DNA was isolated and 300 ng of genomic DNA was digested with APE1 endonuclease (cat # M0282S, New England Biolabs), AAG glycosylase (cat # M0313S, New England Biolabs), or both enzymes in MOPS

(17) CHD4 Conceals Aberrant CTCF-Binding Sites at TAD Interiors by Regulating Chromatin Accessibility in Mouse Embryonic Stem CellsMolecules and CellsNovember 30, 2021Sungwook Han, Hosuk Lee, Andrew J. Lee, Seung-Kyoon Kim, Inkyung Jung, Gou Young Koh, Tae-Kyung Kim, Daeyoup Leeselected cDNAs were circularized using CircLigase (CL4111K; Epicentre, USA) and re-linearized with ApeI (M0282S; NEB). The single-stranded DNA templates were amplified using Phusion High-Fidelity DNA Polymerase (M0530S;

(18) Click and Cut: a click chemistry approach to developing oxidative DNA damaging agentsNucleic Acids ResearchOctober 11, 2021Natasha McStay, Creina Slator, Vandana Singh, Alex Gibney, Fredrik Westerlund, Andrew Kellettenzymes—Fpg (M0240S), Endo III (M0268S), Endo IV (M0304S), Endo V (M0305S), hAAG (M0313S) and APE1 (M0282S)—were purchased from New England Biolabs. Aminoallyl-dUTP-ATTO-647N and YOYO-1 were purchased from Jena

(19) XRCC1 prevents toxic PARP1 trapping during DNA base excision repairMolecular cellJuly 15, 2021Demin AA, Hirota K, Tsuda M, Adamowicz M, Hailstone R, Brazina J, Gittens W, Kalasova I, Shao Z, Zha S, et al.(Adh),Sigma-Aldrich,Cat#A3263 PARG enzyme,Trevigen,N/A Exonuclease III,NEB,Cat#M0206S APE1,NEB,Cat#M0282S Uracil-DNA Glycosylase (UDG),NEB,Cat#M0280S Recombinant PARP1,This paper,N/A Recombinant XRCC1,"This

(20) Functional coordination of BET family proteins underlies altered transcription associated with memory impairment in fragile X syndromeScience AdvancesMay 21, 2021Seung-Kyoon Kim, Xihui Liu, Jongmin Park, Dahun Um, Gokhul Kilaru, Cheng-Ming Chiang, Mingon Kang, Kimberly M. Huber, Keunsoo Kang, Tae-Kyung KimEpicentre, CL4111K) for 2 hours at 60°C and relinearized at the basic dSpacer furan with Ape 1 (15 U; NEB, M0282) for 2 hours at 37°C. The relinearized single-stranded DNA template was subjected to PCR amplification

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APE 1

APE 1 from New England Biolabs

Description

Specifications/Features

New England Biolabs

Citations (53)

Reviews

APE 1 from New England Biolabs

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